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Rmd ee108a9 LucianoRogerio 2021-10-15 Update of the trials of a Breeding program per year
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Rmd 5d56695 LucianoRogerio 2021-10-11 Add of the First Branch height measure at 9 MAP and new table for the number of trials for each Institute
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html 0d2341c LucianoRogerio 2021-09-09 Small changes at writing
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Rmd 7f7b622 LucianoRogerio 2021-07-22 Update PhenDataRmd
html 7f7b622 LucianoRogerio 2021-07-22 Update PhenDataRmd
Rmd 6d25535 LucianoRogerio 2021-07-22 Update PhenDataRmd
html 6d25535 LucianoRogerio 2021-07-22 Update PhenDataRmd
Rmd 3e1bc0d LucianoRogerio 2021-07-22 PhenD update
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html d25ce40 LucianoRogerio 2021-07-22 Build site.
Rmd 73f4e1f LucianoRogerio 2021-07-22 wflow_publish("analysis/PhenData.Rmd")
html ce3ca50 LucianoRogerio 2021-07-21 Build site.
Rmd e7db3e0 LucianoRogerio 2021-07-21 Publish the update 1 Phenotypic data CassavaArchitectureGP

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Phenotypic data - Cassavabase

Initialy we are going to download phenotypic data from Cassavabase. In this case we are going to download the data from traits related to plant Architecture, as the following table:


Table 1. List of traits downloaded from Cassavabase.

Trait Abbreviation Cassavabase code Description
plant architecture visual rating 1-5 PlantArchitecture CO_334:0000099 Plant architecture on a 1-5 scale with 1 = excellent, 2 good, 3 = fair, 4 = bad, and 5 = very bad
plant architecture visual rating 1-5 at month 8 PlantArchitecture8m COMP:0000119 Plant architecture on a 1-5 scale with 1 = excellent, 2 good, 3 = fair, 4 = bad, and 5 = very bad
flowering ability visual assessment 0-3 FlowerVisRate CO_334:0000233 Presence of flowers (0=none; 1=little; 2=intermediate; 3=many).
flower visual rating 0 & 1 FlowerPresence CO_334:0000111 Visual rating of flowers (50%) in plant with 0 = absent and 1 = present.
initial plant vigor assessment 1-5 VigorCIAT CO_334:0000220 Visual assessment of plant vigor during establishment (1=very little vigor, and 5 = very vigorous). as being evaluated by CIAT.
initial vigor assessment 1-7 Vigor CO_334:0000009 Visual assessment of plant vigor during establishment scored one month after planting. 3 = Not vigorous, 5 = Medium vigor, 7 = highly vigorous.
number of forks counting NFC CO_334:0000146 Number of branches (2 forks/branches (dichotomous), 3 forks/branches (trichotomous), or 4 forks/branches (tetrachotomous)) at every branching level.
number of forks on branching level 1 counting NFC1 CO_334:0000522 Number of forks (2 forks/branches (dichotomous), 3 forks/branches (trichotomous), or 4 forks/branches (tetrachotomous)) on the first branching level.
number of forks on branching level 2 counting NFC2 CO_334:0000523 Number of forks (2 forks/branches (dichotomous), 3 forks/branches (trichotomous), or 4 forks/branches (tetrachotomous)) on the second branching level.
number of forks on branching level 3 counting NFC3 CO_334:0000524 Number of forks (2 forks/branches (dichotomous), 3 forks/branches (trichotomous), or 4 forks/branches (tetrachotomous)) on the third branching level.
number of nodes at branching level 1 counting NNC1 CO_334:0000352 Number of nodes at the first branching level.
number of nodes at branching level 2 counting NNC2 CO_334:0000363 Number of nodes at the second branching level.
number of nodes at branching level 3 counting NNC3 CO_334:0000368 Number of nodes at the third branching level.
first apical branch height measurement in cm FirstBranchHeight CO_334:0000106 Height of first apical branch (ground level to point of first Apical branch, 9 months after planting) in cm
plant height measurement in cm PlantHeight CO_334:0000018 Vertical height of plants from the ground to top of the canopy measured in centimeter (cm).
plant height measurement in cm at month 12 PlantHeight12m COMP:0000181 Vertical height of plants from the ground to top of the canopy measured in centimeter (cm).
plant height with leaf in cm PlantHeightLeaf CO_334:0000123 Portion of the stem with leaves measured as the distance in centimeter from the point of attachment of the oldest leaf to the youngest leaf (apical leaf portion).
plant height without leaf PlantHeightNLeaf CO_334:0000125 Portion of stem with no leaf measured in centimeter (cm) by deducting plant height with leaf from plant height.
plant height without leaf at month 12 PlantHeightNLeaf12m COMP:0000182 Portion of stem with no leaf measured in centimeter (cm) by deducting plant height with leaf from plant height.
stalk length evaluation StalkLength CO_334:0000227 Visual assessment of the average length of the stalks (1=short; 2=intermediate; 3=long)
stem diameter measurement in cm StemDiam CO_334:0000257 Measurement of stem diameter taken on the middle of the plant in centimeter (cm) using the vernier caliper.
stem diameter measurement in cm at month 5 StemDiam5m COMP:0000129 Measurement of stem diameter taken on the middle of the plant in centimeter (cm) using the vernier caliper.
stem diameter measurement in cm at month 6 StemDiam6m COMP:0000130 Measurement of stem diameter taken on the middle of the plant in centimeter (cm) using the vernier caliper.


1. Download of the phenotypic dataset from Cassavabase wizard tool

Use the following filters:

  1. Select the trait list TraitsLucianoGS
  2. Select the following Trial Types:
    • Clonal Evaluation;
    • Preliminary Yield Trial;
    • Advanced Yield Trial;
    • Uniform Yield Trial;
    • Regional Trials;
    • phenotyping_trial.
  3. Select Years from 2010 to 2021.
  4. Select all the trials available at cassavabase after the filter.

2. Phenotypic data Editing

library(tidyverse); library(data.table); library(here)

RawPhenoData <- read.csv(file = here::here("data", "phenodata.csv"), header = T, na.strings = "")

RawPhenoData %>% mutate("FlowerPresence" = flower.visual.rating.0.1.CO_334.0000111,
                        "FlowerVisRate" = flowering.ability.visual.assessment.0.3.CO_334.0000233,
                        "VigorCIAT" = initial.plant.vigor.assessment.1.5.CO_334.0000220,
                        "Vigor" = initial.vigor.assessment.1.7.CO_334.0000009,
                        "PlantArchitecture" = plant.architecture.visual.rating.1.5.CO_334.0000099,
                        "PlantHeight" = plant.height.measurement.in.cm.CO_334.0000018,
                        "PlantHeight12m" = plant.height.measurement.in.cm.month.12.COMP.0000181,
                        "PlantHeightLeaf" = plant.height.with.leaf.in.cm.CO_334.0000123,
                        "PlantHeightNLeaf" = plant.height.without.leaf.CO_334.0000125,
                        "PlantHeightNLeaf12m" = plant.height.without.leaf.month.12.COMP.0000182,
                        "StalkLength" = stalk.length.evaluation.CO_334.0000227,
                        "StemDiam" = stem.diameter.measurement.in.cm.CO_334.0000257,
                        "FirstBranchHeight" = first.apical.branch.height.measurement.in.cm.CO_334.0000106,
                     .keep = "unused") %>% melt(data = .,
                                                id.vars = c("studyYear", "programDbId", "programName", "programDescription",
                                                            "studyDbId", "studyName", "studyDesign", "plotWidth",
                                                            "plotLength", "fieldSize", "fieldTrialIsPlannedToBeGenotyped",
                                                            "fieldTrialIsPlannedToCross", "plantingDate", "harvestDate",
                                                            "locationDbId", "locationName", "germplasmDbId", "germplasmName",
                                                            "germplasmSynonyms", "observationLevel", "observationUnitDbId",
                                                            "observationUnitName", "replicate", "blockNumber", "plotNumber",
                                                            "rowNumber", "colNumber", "entryType", "plantNumber"),
                                                variable.name = "Trait",
                                                value.name = "Value") -> PhenoData
PhenoData2 <- PhenoData[!is.na(PhenoData$Value),]

saveRDS(object = PhenoData2, file = here::here("data", "phenotypePAGP.RDS"))
rm(list = ls())

3. Phenotypic data Information

suppressMessages(library(tidyverse)); library(reactable); library(here)
PhenoData <- readRDS(here("data","phenotypePAGP.RDS"))


PhenoData %>% dplyr::select(programName, studyYear, Trait, studyName) %>% unique() -> PhenoData2

Table2 <- table(PhenoData2$programName, PhenoData2$studyYear, PhenoData2$Trait) %>% as.data.frame()
colnames(Table2) <- c("programName", "Year", "Trait", "N˚Trials")
Table2 <- Table2[Table2$`N˚Trials`!=0,]

Table 2. Number of trial per Institute in Cassavabase with Cassava Plant Shape Traits.

Table 3. Plot number of the trials available in Cassavabase with Cassava Plant Shape traits.

4. Heritability and Reliability analysis per trial

library(plyr)
source(here::here("code", "MixedModelsFunctions.R"))

PhenoData$check <- ifelse(PhenoData$entryType!="test", PhenoData$germplasmName, "999") 
PhenoData$new <- ifelse(PhenoData$entryType == "test", 1, 0) 

PhenoData$blockNumber <- as.character(PhenoData$blockNumber)
#PhenoData2 <- PhenoData[!PhenoData$studyName %in% BadTrialsList,]


#fmfit <- PhenoData2 %>% filter(Trait == "PlantArchitecture") %>% dlply(.variables = c("Trait", "studyName"),
#                              .fun = analyzeTrial.sommer)

The heritability and Reliability were estimated for each trial. We will proceed with the trails with reliability and heritability bigger than 0.2 and 0.1, respectively.

Select the trials with unexpected number of blocks, controls for their experimental design

PhenoData %>% dplyr::group_by(programName, studyYear, studyDesign, studyName) %>%
  dplyr::summarise(n_blocks = length(unique(blockNumber)),
                   n_reps = length(unique(replicate)),
                   controls = length(unique(check))) -> TrialsInfo
`summarise()` has grouped output by 'programName', 'studyYear', 'studyDesign'. You can override using the `.groups` argument.
TrialsInfo %>% dplyr::filter((studyDesign %in% c("Augmented", "Alpha", "RCBD") & n_blocks == 1) |
                               (studyDesign == "Augmented" & controls == 1)) -> BadTrialsInfo
BadTrialsList <- BadTrialsInfo$studyName

Table 4. Number of blocks, replicates and control of the trials available in Cassavabase with Cassava Plant Shape traits.

Table 5. Trials available in Cassavabase with Cassava Plant Shape traits with strange metadata.

Next Steps

  • Download Phenotypic dataset;
  • select trials by reliability and heritability;
  • estimate the BLUPS, and genetic correlations between the traits;
  • Create a list with the clone names;
  • Download the Genotypic dataset from the clones phenotyped;
  • Perform the genomic prediction single-trait with G-BLUP Add, Add + Dom genetic models. - 50 replicates - 100 clones per fold.

home


sessionInfo()
R version 4.1.1 (2021-08-10)
Platform: aarch64-apple-darwin20 (64-bit)
Running under: macOS Big Sur 11.6.1

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.1-arm64/Resources/lib/libRblas.0.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.1-arm64/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] lme4_1.1-27.1   sommer_4.1.5    crayon_1.4.2    lattice_0.20-45
 [5] MASS_7.3-54     Matrix_1.4-0    plyr_1.8.6      here_1.0.1     
 [9] reactable_0.2.3 forcats_0.5.1   stringr_1.4.0   dplyr_1.0.7    
[13] purrr_0.3.4     readr_2.1.1     tidyr_1.1.4     tibble_3.1.6   
[17] ggplot2_3.3.5   tidyverse_1.3.1

loaded via a namespace (and not attached):
 [1] httr_1.4.2        sass_0.4.0        jsonlite_1.7.2    splines_4.1.1    
 [5] modelr_0.1.8      bslib_0.3.1       assertthat_0.2.1  cellranger_1.1.0 
 [9] yaml_2.2.1        pillar_1.6.4      backports_1.4.1   glue_1.6.0       
[13] digest_0.6.29     promises_1.2.0.1  minqa_1.2.4       rvest_1.0.2      
[17] colorspace_2.0-2  htmltools_0.5.2   httpuv_1.6.5      reactR_0.4.4     
[21] pkgconfig_2.0.3   broom_0.7.11      haven_2.4.3       scales_1.1.1     
[25] whisker_0.4       later_1.3.0       tzdb_0.2.0        git2r_0.29.0     
[29] generics_0.1.1    ellipsis_0.3.2    withr_2.4.3       cli_3.1.0        
[33] magrittr_2.0.1    readxl_1.3.1      evaluate_0.14     fs_1.5.2         
[37] fansi_0.5.0       nlme_3.1-153      xml2_1.3.3        tools_4.1.1      
[41] hms_1.1.1         lifecycle_1.0.1   munsell_0.5.0     reprex_2.0.1     
[45] compiler_4.1.1    jquerylib_0.1.4   rlang_0.4.12      nloptr_1.2.2.3   
[49] grid_4.1.1        rstudioapi_0.13   htmlwidgets_1.5.4 crosstalk_1.2.0  
[53] rmarkdown_2.11    boot_1.3-28       gtable_0.3.0      DBI_1.1.2        
[57] R6_2.5.1          lubridate_1.8.0   knitr_1.37        fastmap_1.1.0    
[61] utf8_1.2.2        workflowr_1.7.0   rprojroot_2.0.2   stringi_1.7.6    
[65] Rcpp_1.0.7        vctrs_0.3.8       dbplyr_2.1.1      tidyselect_1.1.1 
[69] xfun_0.29